31
3.2. Application of proposed methods to real
sample
To verify the potential application of proposed
method to real samples, three real wastewater
samples were selected and used by proposed
procedure. Different concentrations of amoxicillin
include 100, 150 and 200 mg L
-1
were spiked to the
samples and removal efciency of amoxicillin was
tested using proposed methods. Results are shown
in Table 3. As expected, the magnetic bentonite
nanocomposite has good recovery for adsorption of
the spiked amoxicillin from all three real samples.
4. Conclusions
In this work, a magnetic bentonite nanocomposite
was synthesized and applied for adsorption of
amoxicillin from wastewater. A central composition
design was applied for study of the effects of
parameters such as; pH, temperature and amount
of adsorbent which was inuenced on the removal
efciency of amoxicillin. A quadratic model was
solved and developed to correlate the independed
variables and the response of system. Through
the analysis of response surfaces, was found that
amount of adsorbent was most signicant variable
on removal efciency of amoxicillin by HPLC.
Process optimization was performed and results
showed that the experimental data were found to
agree with the predicted values.
5. References
[1] T.H. Le, C. Ng, Removal of antibiotic
residues, antibiotic resistant bacteria and
antibiotic resistance genes in municipal
wastewater by membrane bioreactor systems,
Water res., 145 (2018) 498-508.
[2] W. Yan, Y. Xiao, W. Yan, The effect of
bioelectrochemical systems on antibiotics
removal and antibiotic resistance genes: a
review, Chem. Eng. J., 358 (2019) 1421-
1437.
[3] S. Ren , C. Boo , N. Guo, Photocatalytic
reactive ultraltration membrane for removal
of antibiotic resistant bacteria and antibiotic
resistance genes from wastewater efuent,
Environ. Sci. Technol., 52 (2018) 8666-8673.
[4] Z. Cao, X. Liu, J. Xu, J. Zang, Removal of
antibiotic orfenicol by sulde-modied
nanoscale zero-valent iron, Environ. Sci.
Technol., 51 (2017) 11269-11277.
[5] N. Li, G.-P. Sheng, Removal of antibiotic
resistance genes from wastewater treatment
plant efuent by coagulation, Water Res., 111
(2017) 204-212.
[6] Y. Zhou, Q. Yang, D. Zhang, N. Gan, Q.
Li, Detection and removal of antibiotic
tetracycline in water with a highly stable
luminescent MOF, Sensors and Actuators B:
Chem., 262 (2018) 137-143.
[7] Y. Hong, C. Li, G. Zhang, Efcient and stable
Nb
2
O
5
modied g-C3N4 photocatalyst for
removal of antibiotic pollutant, Chem. Eng.
J., 299 (2016) 74-84.
[8] C. Guo, H
2
O
2
and/or TiO
2
photocatalysis
under UV irradiation for the removal
of antibiotic resistant bacteria and their
antibiotic resistance genes, J. Hazard. Mater.,
323 (2017) 710-718.
[9] C. Hong, P.-Y. Hong, Removal of antibiotic-
resistant bacteria and antibiotic resistance
genes affected by varying degrees of fouling
on anaerobic microltration membranes,
Environ. Sci. Technol., 51 (2017) 12200-
12209.
[10] B. Kayan, B. Gözmen, Degradation of Acid
Red 274 using H2O2 in subcritical water:
Application of response surface methodology,
J. Hazard. Mater., 201 (2012) 100-106.
[11] S. Tang, D. Yuan, Y. Rao, N. Li, Persulfate
activation in gas phase surface discharge
plasma for synergetic removal of antibiotic in
water., Chem. Eng. J., 337 (2018) 446-454.
[12] F.S. Hashem, Removal of methylene blue by
magnetite covered bentonite nano-composite,
Eur. Chem. Bull., 2 (2013) 524-529.
Magnetic bentonite nanocomposite for removal of amoxicillin Mohammad Reza Rezaei Kahkha et al