Determination of mercury values in urine and air of chloralkali workers by copper nanoparticles functionalized in carboxylic carbon nanotubes and the effects of mercury exposure on oxidative stress

Volume 5, Issue 02, Pages 76-89, Jun 2022 *** Field: Analytical Method in Environment and human

  • Ali Faghihi Zarandi Department of Occupational Health, School of Public Health, Kerman University of Medical Sciences, Kerman, Iran
  • Somayyeh Karami-Mohajeri Pharmaceutics Research Center, Institute of Neuropharmacology, Kerman University of Medical Sciences, Kerman, Iran
  • Morteza Mehdipour Rabouri Department of Occupational Health, School of Public Health, Kerman University of Medical Sciences, Kerman, Iran
  • Abbas Mohammadhosseini- Heyran Department of Occupational Health, School of Public Health, Kerman University of Medical Sciences, Kerman, Iran
  • Zahed Ahmadi, Corresponding Author, Department of Occupational Health Engineering, School of Public Health, Iranshahr University of Medical Sciences, Iranshahr, Iran
Keywords: Mercury, Air and Urine, Magnetic solid-phase extraction, Oxidative stress, Copper functionalized carbon nanotubes, Cold vapor atomic absorption spectrometer


Mercury exposure can produce toxic organic compounds in the body. Also, mercury can potentially cause oxidative damage and cellular disorders. In this study, the determination of mercury values in urine and air of chloralkali workers based on copper nanoparticles functionalized in carboxylic carbon nanotubes (CuNPs@CNT-COOH) were obtained by cold vapor atomic absorption spectrometer (CV-AAS). The urine samples were determined by magnetic solid-phase extraction (MSPE) at pH 8.0. By measuring the mercury level in the air and the urine sample of workers, the level of oxidative stress (Malondialdehyde (MDA), Superoxide Dismutase (SOD) and Catalase (Cat)), Interleukin-6 (IL-6), and Tumor Necrosis Factor α (TNF-α) as the proinflammatory cytokines were measured in the subject group. The results revealed statistically significant differences in the mercury level of the urine samples in the case and control groups (p<0.001). Similarly, the malondialdehyde (MDA) level was significantly different between the two research groups (p<0.001). Catalase concentration was not significantly different in the two groups (p=0.059). The LOD and linear range for mercury determination in urine were achieved at 0.012 µg L−1 and 0.05-7.0 µg L−1, respectively. Workers’ exposure to mercury can significantly increase oxidative stress and inflammatory cell signaling molecules such as cytokines.


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How to Cite
Faghihi Zarandi, A., Karami-Mohajeri, S., Mehdipour Rabouri, M., Mohammadhosseini- Heyran, A., & Ahmadi, Z. (2022). Determination of mercury values in urine and air of chloralkali workers by copper nanoparticles functionalized in carboxylic carbon nanotubes and the effects of mercury exposure on oxidative stress. Analytical Methods in Environmental Chemistry Journal, 5(02), 76-89.
Original Article