Food Analysis: Task specific ionic liquids for separation of nickel and cadmium from olive oil samples by thermal ultrasound-assisted dispersive multiphasic microextraction

Vol 2, Issue 02, Pages 55-64,*** Field: Food Analysis

  • Asian Khaligh Semnan university
Keywords: Olive oil, Cadmium and nicke, Thermal ultrasound-assisted dispersive multiphasic microextraction, Task specific ionic liquid, Atom trap flame atomic absorption spectrometry


A novel task-specific ionic liquid (TSILs) was used for highly sensitive extraction and separation of nickel and cadmium in olive oil by thermal ultrasound-assisted dispersive multiphasic microextraction (TUSA-DMPμE). By proposed method, a mixture containing of hydrophilic TSILs (α- Cyano-4-hydroxycinnamic acid diethylamine; [CHCA] [DEA] and 1-(2-Hydroxyethyl)-3-methylimidazolium tetrafluoroborate; [HEMIM][BF4]) as a complexing and extracting solvent, acetone as a dispersant of TSILs was added to diluted olive oil with n-hexane containing Cd (II) and Ni (II) that was already complexed by TSILs in 60OC at pH 6.0-7.5. After optimized conditions, the enrichment factor (EF), Linear range (LR) and limit of detection (LOD) were obtained (19.3; 19.6), (5.0- 415 μg L-1; 2.7- 92 μg L-1) and (1.3 μg L-1;  0.6 μg L-1) with [CHCA] [DEA] and (13.7; 14.2), (7.5- 600 μg L-1; 3.6- 128 μg L-1) and (2.2 ng L-1; 0.9 μg L-1) with [HEMIM][BF4] for Ni and Cd ions in olive  samples respectively.


O. Acar, Evaluation of cadmium, lead, copper, iron and zinc in Turkish dietary vegetable oils and olives using electrothermal and flame atomic absorption spectrometry, grasas y aceites, 63 (2012) 383-393.

A. Palizban, A. Badii, G. Asghari, H. Mardani-Nafchi, Lead and cadmium contamination in seeds and oils of Brassica napus L and Carthamus tinctorius grown in Isfahan Province/Iran, Iranian J. Toxicol., 8 (2015) 1196-1202.

E.K. Baran, S.B. Yaşar, Zinc and nickel determination in liquid edible oils by FAAS after the extraction, Eur. j. lipid sci. technol., 114 (2012) 1320-1326.

K. Bakkali, N.R. Martos, B. Souhail, E. Ballesteros, Determination of heavy metal content in vegetables and oils from Spain and Morocco by inductively coupled plasma mass spectrometry, Anal. Lett., 45 (2012) 907-919.

M.Y. Aşci, A. Efendioglu, B. Bati, Solid phase extraction of cadmium in edible oils using zinc piperazinedithiocarbamate and its determination by flame atomic absorption spectrometry, Turkish J. Chem., 32 (2008) 431-440.

C. Onyema, K. Ibe, Effects of Refining Processes on the Physicochemical Properties of Some Selected Vegetable Oils, Am. Chem. Sci. J., 12 (2016) 1-7.

S.U. Okorie, C.N. Nwachukwu, Comparative Evaluation Of Quality Characteristics Of Oils Extracted From Some Selected Legumes And A Cereal, IOSR J. Environ. Sci., Toxicol. Food Technol., 8 (2014) 66-69.

H. Well, Agency for Toxic Substances and Disease Registry (ATSDR), 2015.

M. Hezbullah, S. Sultana, S. Chakraborty, M. Patwary, Heavy metal contamination of food in a developing country like Bangladesh: An emerging threat to food safety, J. Toxicol. Environ. Health Sci., 8 (2016) 1-5.

F. Zhu, W. Fan, X. Wang, L. Qu, S. Yao, Health risk assessment of eight heavy metals in nine varieties of edible vegetable oils consumed in China, Food chem. toxicol., 49 (2011) 3081-3085.

D. Mendil, Ö.D. Uluözlü, M. Tüzen, M. Soylak, Investigation of the levels of some element in edible oil samples produced in Turkey by atomic absorption spectrometry, J. Hazard. Mater., 165 (2009) 724-728.

M. Zeiner, I. Steffan, I.J. Cindric, Determination of trace elements in olive oil by ICP-AES and ETA-AAS: A pilot study on the geographical characterization, Microchem. J., 81 (2005) 171-176.

I.J. Cindric, M. Zeiner, I. Steffan, Trace elemental characterization of edible oils by ICP–AES and GFAAS, Microch. J., 85 (2007) 136-139.

E. Llorent-Martínez, P. Ortega-Barrales, M. Fernández-de Córdova, A. Domínguez-Vidal, A. Ruiz-Medina, Investigation by ICP-MS of trace element levels in vegetable edible oils produced in Spain, Food Chem., 127 (2011) 1257-1262.

T.G. Díaz, A. Guiberteau, M.L. Soto, J. Ortiz, Determination of copper with 5, 5-dimethylcyclohexane-1, 2, 3-trione 1, 2-dioxime 3-thiosemicarbazone in olive oils by adsorptive stripping square wave voltammetry, Food Chem., 96 (2006) 156-162.

F. Tokay, S. Bağdat, Spectrometric determination of iron and copper in vegetable oils after separation with Schiff base impregnated silica gel column: A simple approach for eliminating the high organic matrix, Int. j. food sci. technol., 50 (2015) 2694-2699.

F. Tokay, S. Bağdat, Determination of iron and copper in edible oils by flame atomic absorption spectrometry after liquid–liquid extraction, J. Am. Oil Chem. Soc., 92 (2015) 317-322.

M. Rezaee, Y. Assadi, M.-R.M. Hosseini, E. Aghaee, F. Ahmadi, S. Berijani, Determination of organic compounds in water using dispersive liquid–liquid microextraction, J. Chromatogr. A, 1116 (2006) 1-9.

I. López-García, Y. Vicente-Martínez, M. Hernández-Córdoba, Determination of cadmium and lead in edible oils by electrothermal atomic absorption spectrometry after reverse dispersive liquid–liquid microextraction, Talanta, 124 (2014) 106-110.

H. Shirkhanloo, A. Khaligh, H.Z. Mousavi, M.M. Eskandari, A.A. Miran-Beigi, Ultra-trace arsenic and mercury speciation and determination in blood samples by ionic liquid-based dispersive liquid-liquid microextraction combined with flow injection-hydride generation/cold vapor atomic absorption spectroscopy, Chem. Papers, 69 (2015) 779-790.

H. Shirkhanloo, M. Ghazaghi, H.Z. Mousavi, Cadmium determination in human biological samples based on trioctylmethyl ammonium thiosalicylate as a task-specific ionic liquid by dispersive liquid–liquid microextraction method, J. Mol. Liq., 218 (2016) 478-483.

J. Werner, Determination of metal ions in tea samples using task‐specific ionic liquid‐based ultrasound‐assisted dispersive liquid–liquid microextraction coupled to liquid chromatography with ultraviolet detection, J. sep. sci., 39 (2016) 1411-1417.

S. Sadeghi, A.Z. Moghaddam, Task-specific ionic liquid based in situ dispersive liquid–liquid microextraction for the sequential extraction and determination of chromium species: optimization by experimental design, RSC Adv., 5 (2015) 60621-60629.

N. Khan, T.G. Kazi, H.I. Afridi, M.B. Arain, Determination of cadmium in human serum and blood samples after dispersive liquid-liquid microextraction using a task specific ionic liquid, Anal. Lett., (2017).

M.J. Trujillo-Rodríguez, P. Rocío-Bautista, V. Pino, A.M. Afonso, Ionic liquids in dispersive liquid-liquid microextraction, TrAC Trends in Anal. Chem., 51 (2013) 87-106.

Q. Zhou, H. Bai, G. Xie, J. Xiao, Temperature-controlled ionic liquid dispersive liquid phase micro-extraction, J. Chromatogr. A, 1177 (2008) 43-49.

Q. Zhou, X. Zhang, J. Xiao, Ultrasound-assisted ionic liquid dispersive liquid-phase micro-extraction: A novel approach for the sensitive determination of aromatic amines in water samples, J. Chromatogr. A, 1216 (2009) 4361-4365.

J. Zhang, H. Gao, B. Peng, S. Li, Z. Zhou, Comparison of the performance of conventional, temperature-controlled, and ultrasound-assisted ionic liquid dispersive liquid–liquid microextraction combined with high-performance liquid chromatography in analyzing pyrethroid pesticides in honey samples, J. Chromatogr. A, 1218 (2011) 6621-6629.

How to Cite
Khaligh, A. (2019). Food Analysis: Task specific ionic liquids for separation of nickel and cadmium from olive oil samples by thermal ultrasound-assisted dispersive multiphasic microextraction. Analytical Methods in Environmental Chemistry Journal, 2(2), 55-64.
Original Article