TY - JOUR AU - Ali Akbar Miran Beigi AU - Mojtaba Shamsipur PY - 2019/03/18 Y2 - 2024/03/29 TI - Biochemistry Method: Simultaneous determination of formaldehyde and methyl tert-buthyl ether in water samples using static headspace gas chromatography mass spectrometry JF - Analytical Methods in Environmental Chemistry Journal JA - AMECJ VL - 2 IS - 01 SE - Original Article DO - 10.24200/amecj.v2.i01.40 UR - http://journal.amecj.com/index.php/AMECJ-01/article/view/40 AB - The present study describes a method based on staticheadspace extraction (HS) followed by gas chromatography/mass spectrometry (GC/MS) for the qualitative and quantitativeanalysis of methyl tert-buthyl ether (MTBE) and formaldehyde(HCHO) in water samples. Cytochrome P4502A6 has importantrole for converting of MTBE to tert-butyl alcohol (TBA) andHCHO. To enhance the extraction capability of the HS, extractionparameters such as extraction temperature, extraction time, theratio of headspace volume to sample volume and sodium chlorideconcentration have been optimized. Wide linearity range wasverified in a range of 5-10000 μgL-1 for MTBE (r2=0.9998), whilethose for HCHO was 5-500 μg L-1 (r2=0.9996). Detection limitsfor MTBE and HCHO was 1.0 μg L-1 and 1.3 μg L-1, respectively.Best results were obtained when the analyzed oily water sampleswere heated to 70 ◦C for 20 min, with the sample volume 10mL in 20 mL vial, and NaCl 30% (w/v) was used to saturate thesamples. The proposed analytical method was successfullyused for the quantification of analytes in water and wastewatersamples. ER -