_1718831285 1718831285 Hamid Shirkhanloo sahar.zi67@gmail.com Hamid Shirkhanloo Analytical Methods in Environmental Chemistry Journal AMECJournal 2645-5382 2645-5552 06 30 2024 7 02 10.24200/amecj.v7.i01 https://journal.amecj.com/index.php/AMECJ-01/issue/view/30 Zefzoufi Manal El Mansouri Az-Eddine Ait El Had Mustapha Bouamama Hafida Belaqziz Majdouline Sammama Amal Outaki Meriem Elmasloumi Mohamed Mohamed Among the natural products, phenolic and alkaloid compounds have the most important constituents and common plant-derived secondary metabolites, and thus, analysis deserves particular attention for their therapeutic proprieties. In this study, the phenolic and alkaloid compounds were analyzed using ultra-high-performance liquid chromatography coupled with diode array detector-tandem electrospray ionization mass spectrometry (UHPLC/DAD/ESI-MS) for three extracts of Moroccan Berberis vulgaris L. Then we optimized and validated results for four phenolic compounds. To evaluate the biological activity of this species we chose the antimicrobial activity. For the antimicrobial activity of these extracts, we used molecular docking analysis of the compounds identified with DNA gyrase. The method validation results showed a good linear (R2>0.99) with the coefficient correlation and the significance of regression tests confirmed a linear relationship between x and y. Two concentrations of phenolic and alkaloid compounds (31.25 mg L-1 and 500 mg L-1) were determined by the UHPLC/DAD/ESI-MS and used in repeatability tests, the relative standard deviation of 10 repetitions varied to 0.35-2.33 with CV% 1.1-2%. In addition, the analytical method is sensitive, accurate, and precise (CV<5%). The analytical method successfully set for robust quality evaluation and standardization of phenolic and alkaloid compounds from B. vulgaris organic extract.   06 29 2024 29 44 http://creativecommons.org/licenses/by/4.0 10.24200/amecj.v7.i02.284 https://journal.amecj.com/index.php/AMECJ-01/article/view/284 https://journal.amecj.com/index.php/AMECJ-01/article/download/284/580 https://journal.amecj.com/index.php/AMECJ-01/article/download/284/580