Dispersive solid phase microextraction based on aminefunctionalized bimodal mesoporous silica nanoparticles for separation and determination of calcium ions in chronic kidney disease
Volume1,Issue01,Pages57-66,Ar-AMC-37 *** Filed: Human Bioanalysis
Abstract
The ultrasound assisted- dispersive solid phase microextraction method (USA-SPME) was used for in-vitro study on separation/extraction of calcium ions in human blood of chronic kidney disease (CKD). In this procedure, amine-functionalized bimodal mesoporous silica nanoparticle (NH2-UVM7) as a solid phase was used for in-vitro separation/extraction of calcium from blood/serum samples. Moreover, a mixture of NH2-UVM7 with ionic liquid and acetone (S/IL/Ac) was added to serum/blood sample containing of Ca (II) at pH of 7.3. After ultrasonic bath and centrifuging, NH2-UVM7/ IL settled down in bottom of tube, which was extracted Ca (II) ions by binding to amine group ([Ca]2+ →: NH2 ─ UVM7). The concentration of Ca (II) was determined by flame atomic absorption spectrometry (F-AAS, N2O, C2H2) after back extraction remained adsorbent in IL by 0.5 mL of HNO3 (0.5 M).
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