Sulfamethizole functionalized graphene oxide for in-vitro separation and determination lead in blood serum of battery manufactories workers by syringe filter-dispersive- micro solid phase extraction

Volume 3, Issue 04, Pages 17-29, Dec 2020 *** Field: Bioanalytical Chemistry

  • Abhijit De, (*Corresponding Author) HITech institute of theoretical and computational chemistry, Shivakote, Hesaraghatta Hobli, Bengaluru, India
  • S. Mojtaba Mostafavi Department of Chemistry, Iranian-Australian Community of Science, Hobart, University of Tasmania, Australia
Keywords: Lead, Human blood/serum, Sulfamethizole, Nano graphene oxide, Syringe filter, Dispersive-micro solid phase extraction procedure


The toxic effect of lead (Pb) causes to anemia and iron deficiency in human body. So, the lead determination in blood/serum samples is very important.  In this study, a novel adsorbent based on sulfamethizole functionalized on nanographene oxide (C3H10N4O2S2-NGO; SM-NGO) was used for extraction of Pb(II) from human blood, serum and plasma samples in battery manufactories workers by SF-D-µ-SPE.  By procedure, 25 mg of SM-NGO mixed with 10 mL of human blood/serum or plasma samples and aspirated by 10 mL of syringe tube. After sonication of samples for 5 min, the Pb ions adsorbed based on sulfur of SM-NGO adsorbent at pH=6 and the solid phase separated by syringe coupled to Millex-FG hydrophobic PTFE membrane (0.2 µm). Then, the lead ions were back-extracted from SM-NGO/PTFE by elution phase with 0.5 mL of nitric acid solution (0.5 M). Finally, the concentrations of Pb(II) ions were determined by AT-FAAS. 


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How to Cite
De, A., & Mostafavi, S. M. (2020). Sulfamethizole functionalized graphene oxide for in-vitro separation and determination lead in blood serum of battery manufactories workers by syringe filter-dispersive- micro solid phase extraction. Analytical Methods in Environmental Chemistry Journal, 3(04), 17-29.
Original Article